https://bose-lab.org/work daily 1.0 2026-02-17 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1681292581492-IG7Y3DF586CUSTHPTLI9/IMG_6325.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1681292900770-WFZ9U1RMDIACQBTDRWPC/research+panel-01.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1681292975991-FSTSPW0MWNHP8VLPQ2B7/IMG_6335.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1681293601250-0POYGQY524NRG14S0XO3/IMG_2216.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1681293703552-AVMHGFEKI4SEVWUYC7NX/IMG_0045.jpg Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1681457497172-PQHN84LUGWU03AHEVQO2/F18F9F8C-A4E8-47FD-897B-BDFA92C3D0F9.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604074982581-0E0U9QVIPNNUS3Z81PJP/IMG_7275.jpg Bose Lab - Functional Genomics Luc sequencing nascent RNA using the Nanopore MinION https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604075360050-2YDAD235TA0IYTMPV2WJ/IMG_6950.jpg Bose Lab - Cryo-EM We’re pretty excited about Cryo-EM https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604076363908-6Y43J901NP6TVZOU8JOF/IMG_5354.jpg Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604077142967-C4PLLCEAF0KTOGD39BLZ/IMG_5992.jpeg Bose Lab - Biochemistry We use classical biochemistry to .... https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/16e96608-d90a-444f-9dd0-31688fe61e02/F18F9F8C-A4E8-47FD-897B-BDFA92C3D0F9.png Bose Lab . https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/ce77dfe5-7145-4676-b2b9-fc9dcb32384c/IMG_0642.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543648281341-6NYYZLHXUCJ4MAEBQHTA/IMG_1882.jpg Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493115393459-DOTRBU5YZSUVMABVJTMP/image-asset.png Bose Lab - RNA Binding to CBP Stimulates Histone Acetylation and Transcription. Daniel A. Bose, Greg Donahue, Danny Reinberg, Ramin Sheikhattar, Roberto Bonasio, Shelley L. Berger. Cell 168, 135–149.e22 (2017) In our most recent paper, we showed how RNA binding to CBP could modulate CBPs enzymatic activity to control histone acetylation and gene expression. The model explains how localised transcription of eRNAs could tailor the activity of sub-populations of CBP bound at enhancers, leading to functional diversity in enhancer activity. (manuscript also available here) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493115537673-S7EBLZ67M08UVRMPK7MB/image-asset.jpeg Bose Lab - Organisation of an activator bound RNA polymerase holoenzyme Daniel Bose, Tillmann Pape, Patricia C. Burrows, Mathieu Rappas, Sivaramesh Wigneshweraraj, Martin Buck, and Xiaodong Zhang. Molecular Cell, 32 (3), 337-46 (2008). PMID 18995832. Part of a long-running collaboration with Prof. Martin Buck FRS, we used cryo-EM to describe the mechanism of the alternate sigma factor, sigma-54, in bacteria. When bound to RNA polymerase (RNAP), Sigma-54 needs to interact with a special activator protein bound at a distal enhancer-like element to initiate transcription. These activators use energy from ATP hydrolysis to re-model RNAP/sigma-54 to initiate transcription, a process that we visualised for the first time. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/f88d87cf-f5bc-466c-93a9-f74f59d64af1/Dan+in+the+lab.png Bose Lab - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493131094808-AEOGEZFVE1OOPK31Z9ZR/Research-link-07.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1497609965938-IGH3LU8F2MZTBHN2HHV8/Research-link-08.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1497609708619-SC6VS4KZF5FV77JHCOWB/Research-link-07.png Bose Lab https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/22bbe414-d6fa-4181-9347-479349b78928/IMG_4334.jpeg Bose Lab - Make it stand out Our team in September 2025 https://bose-lab.org/gallery daily 0.75 2020-09-22 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600807507206-RWI91ECUB4IGJBSB67JA/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600807507206-RWI91ECUB4IGJBSB67JA/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572332607850-9XEPBBPVDA2RH9B43NJN/IMG_9970.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572332759406-EGS38LFCS0H1Y8ZJLNOI/IMG_4341.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572332727282-LL18YBR4BO8P15BN1RK6/IMG_5379.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572332797219-PF1YWW8INJSZTF7O48L1/IMG_4392.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600805874347-5HE89L066U8DS1TZND7Y/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600805991816-KJFLFL261QDKWXM4D3PE/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806034204-G2GNZ6SRGYTKW8H49BUS/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806078488-7LVS4OGYJW766SRR4KPM/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806121265-F4I47Q039D4QAC414BBS/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806174871-XAETUUS2OXY13R0WV9FM/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806290089-HIZJ5DEZ72KOOORNJH2W/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806353678-S2W7V2VZJ6KUU8MXCP2D/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806432399-3KB81IU5RJM2O10ZQYDB/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806507032-9MEVWDXMVP1VN0EK8QKE/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806575535-12SPDKI5LGO0KNHJ9BGK/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806662332-YHC52MD79CTW69K691BC/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806743218-D8U81DVVZ4ZUW66H6JP8/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806797590-XS77F4A1E5XSJXQY8FIG/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806840611-TPCM1NGC3H7WAO2PX6OS/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806873536-XUQHPIS2GFCNIQ4BBOFL/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806938450-LB3SWWAT52XWQLZ1YIXM/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600806973082-MHABSA0SRN3UDS0G4ZND/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600807036956-OPP8BZ8CES4UZQ9LRJ2E/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1600807160881-3BSTIS5KE5VE31NN5KJL/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608453554-BYZZV92XUFKXMB12UFQW/IMG_1402.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572332689203-AVLTCYGW9ZX9GPQN6YKY/IMG_3678.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608388458-C8WPYBEVEABQI80LTTR0/IMG_1236.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572332557922-KIH6CHK37PGCR6MDLX12/IMG_2201.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572332637035-XTY9HG3TM15G4HIYI0QC/IMG_2537.jpeg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416494901-ZLDQHBD5YS0U0PJBBOOV/IMG_4966.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416472108-1KGG024OOR2860DH8HL6/IMG_4951.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1494426697718-O54WXA1F4K0HLJYH1EBA/Firth_court_May2017.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608463185-6LQ6BLINZAXYVRYGHCS8/IMG_1510.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493032260746-KQUBTTT23HMZAY0Z4NL4/Filter-binding.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493125618174-CX8R9NDBBBT9H548CZYT/IMG_1062_1.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493032263260-9WUWAJVER3D565295GK2/Join-us.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493038737560-ZTN7UQNYAB40I8ZR41QD/IMG_1941.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493032260636-IRR3LR081RMAGNZA8BS6/Firth-Court.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493038752206-R5CKSJX2BPNMTR8DK3M7/IMG_2217.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493040985419-AH0IFHOGNUMQL6LRFCO9/Social-Logo-03.png Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493041185243-NKOHAH0RERVPAFP59GNM/Graphical+Abstract-02.png Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493132865777-T3VMF3SDIQIRI0I5WTBN/Enhancers-chromatin-06.png Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416264064-UNAATECPGHNXG5Y1APFN/IMG_2820.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416283836-UCZB5S99LUXDC1D0DVY1/IMG_2823.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416303006-1LXV6PBKSABQELHTZX8N/IMG_2911.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416349383-U8I7AIC7I1BU90UT91OQ/IMG_3974.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416377004-0HTNXAHQ24F6FNPXEYDZ/IMG_3993.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416401218-M96JHUY5X9RRDZHRW74Q/IMG_4258.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416413839-3TZVIPNKEM8RKK2P449Z/IMG_4335.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416435277-OH94S21NO30U0BVZUPIX/IMG_4554.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1508416448069-HELNG9GOJXTBT61I9KLU/IMG_4560.JPG Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1513938744042-AGDMDV5XQTWNK80MMVH1/IMG_0209.jpg Gallery - Firth Court at dusk https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876611463-2DVC4VVTDXSWGTF2VPTC/4DB1BA3D-5D12-4223-B4E0-4D61D9333865.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876614537-20XTXACZYVXB395XRNFJ/IMG_0022.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876618662-7P6Q0VXYRSXNRLUVZ71U/IMG_0023.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876626347-5FIHO0CEUR1NHO1C5NOE/IMG_0044.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876628402-JD7HFH2N5KQGCDS7S2WO/IMG_0045.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876638547-OQ5EMEAHXY2FHG7OMVE4/IMG_0273.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876640775-U2JI1CKQ2T0LRS0KGDDU/IMG_0307.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876648421-LDLISKSFPVO0IR5M6H5Z/IMG_0354.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876649046-7HOPE1T54BGBII0NGDOB/IMG_0460.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876655688-P1MOEPB3JML0WQEKKWL6/IMG_0470.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876656220-W4M4MR2K5OBPJW6203F9/IMG_0473.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876663396-BKV9FOQ0Q37REDA8U86E/IMG_0493.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876662401-KYSHKKO20Y0EZMT7RUKY/IMG_0497.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876669923-H10QFVQ8DJ7ZO2OU66UD/IMG_0499.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876685028-YD04QRHH7Z8PJSAHYSWB/IMG_0632+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876694717-UZCYOQJS0M5ADRR1KP1H/IMG_0634+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876699073-EDZTSD5T99PZLN6LBHYA/IMG_0637+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876707938-D5NZ3H5RXWT5G5J783YS/IMG_0638+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876709290-OR3UATKKLLAHSU1GUG1H/IMG_0648+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876724433-GKNIQKNCTD34W75UX78H/IMG_0649+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876716371-N1P9AE47VBTFUKT0XYCC/IMG_0650+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876737900-CFX2R2DEXCULQVBKOXBF/IMG_0690+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876734977-ASYU2GMGGI8YC2QRIIZ5/IMG_0723+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521876745230-PADHHXT56K4HIJCFSDHS/IMG_0725+%281%29.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543607511626-SXSRXHR51I8N1SAI4U7X/IMG_1626.jpg Gallery - Post Viva with Naimah https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608280446-9UQEN80MMGCHO1HWHMI4/IMG_1152.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608340689-3H1MYXGYS57C4KR4P0RU/IMG_1259.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608442961-J6RWXH9GO5LO1RHRHEYE/IMG_1329.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608441319-JSVMXT364Z8D2Q2IJK83/IMG_1357.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608449570-FPFGF9ALN45D041B0ODX/IMG_1477.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608464377-UY94LM1C2SFU2RNC4SKR/IMG_1585.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608476377-0Y8EOTEOWLHJIVC1F1OY/IMG_1589.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608475898-5O3QH6MKR75KX0XPX116/IMG_1734.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543608489190-UL14NT812T6C1MCBISPT/IMG_1910.jpg Gallery https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1544728422556-ARSLKM6EUKE3GSI8NDB0/IMG_2038.jpeg Gallery https://bose-lab.org/blog daily 0.75 2021-11-05 https://bose-lab.org/blog/2021/11/5/starting-up-in-science-nature monthly 0.5 2021-11-05 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/f88d87cf-f5bc-466c-93a9-f74f59d64af1/Dan+in+the+lab.png Lab Blog - Starting up in science: Nature - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/bbd86f2a-17de-4029-91ed-741a5afdd32f/Untitled.png Lab Blog - Starting up in science: Nature - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://bose-lab.org/blog/2021/9/4/shutting-up-for-lockdown monthly 0.5 2021-09-04 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630747996860-JGXP88HDINLNHAQ486X8/IMG_8504.jpeg Lab Blog - Shutting up for lockdown - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://bose-lab.org/blog/2021/9/4/a-new-tissue-culture monthly 0.5 2021-09-04 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745900476-8M0981OS5XTZJ94QM28H/IMG_4350.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745902660-U0ZEO3AT96UJQYRKOCFW/IMG_4351.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745903667-1FNY6QIBN3X76O88EYTU/IMG_4355.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745904756-EA49TMOGRQ604AM8ZW0K/IMG_4361.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745908430-0M2XNI9GWCU0G9S1UOSW/IMG_4376.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745909245-2Q0P6J5TQEUOTFT3WLF3/IMG_5305.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745912845-Y1QGUNLOUGBGSJVWUXRF/IMG_5332.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745913353-JQQOJQ4UMI7U900CBFXA/IMG_5339.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745920627-GF6LMBXWK9PSUGSRH6DW/IMG_5348.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745926398-YXQXUFF2UKM9RKBANKS3/IMG_5379.jpeg Lab Blog - A new Tissue Culture! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1630745927217-1TVVLA6NY7CKLTZN1SNK/IMG_5864.jpeg Lab Blog - A new Tissue Culture! https://bose-lab.org/blog/2019/10/23/were-getting-bigger monthly 0.5 2019-10-23 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1571819720321-6HB5L68PZR3AKDH2UU7P/IMG_5379.jpeg Lab Blog - We're getting bigger! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1571819673129-786STY88PEU44H6N6744/IMG_5296.jpeg Lab Blog - We're getting bigger! https://bose-lab.org/blog/2019/2/5/its-been-a-long-old-year-since-my-last-post monthly 0.5 2019-02-05 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1549370900844-THGANWTVEKN09GH5UZ1R/IMG_2201.jpg Lab Blog - It's been a LONG old year since my last post! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1549370748639-G1FCY34CUZO3A49XQZ5D/IMG_5403.JPG Lab Blog - It's been a LONG old year since my last post! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1549370796275-L5AWQDT4S0VTH5S451BT/IMG_1329.jpg Lab Blog - It's been a LONG old year since my last post! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1549370838241-6W548MV4R6J6VIU5PHWY/IMG_1734.jpg Lab Blog - It's been a LONG old year since my last post! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1549371131236-RS9CBEE08ZRYF8APSUAE/IMG_1510.jpg Lab Blog - It's been a LONG old year since my last post! https://bose-lab.org/blog/2018/3/24/a-winter-blast-then-spring-or-not monthly 0.5 2018-03-24 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874399539-W0MMV2FOI04IXY3UACHX/IMG_0634+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874392839-NLJLDJ615MEZY0DY00J4/IMG_0632+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874407369-DZ344Q5IRB9L6EY0AGSN/IMG_0637+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874413271-6G0JR6VYWW3S6RWQUK25/IMG_0638+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874418416-AUPWR0ABJ9DR3P20AI4X/IMG_0648+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874430809-NAIIR05F4HFTN1XXWGKY/IMG_0649+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874425994-IB1V8164YWSVGJ1JK37W/IMG_0650+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874451832-69KY7T1OWL4KW9ZJ5IRB/IMG_0690+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1521874443594-YZHQXHLCS0JMPPJWS76C/IMG_0723+%281%29.jpg Lab Blog - A winter blast, then spring? (Or not) https://bose-lab.org/blog/2017/12/19/christmas monthly 0.5 2018-02-05 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540828655-90BPIX4JMTC02VUI2JSQ/IMG_0254.jpg Lab Blog - Christmas! Christmas trees in Firth Court https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540903212-3TNXP08LJ0NZPC0Y1G90/IMG_0254.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540903592-732IXNDZFSP87BG34TC0/IMG_0255.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540924006-LH24QGRK4SVCG3XQ0EZW/IMG_0296.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540918868-Y1Y41N64O03DKZV4KSH6/IMG_0307.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540932082-MJ0U4NYJTQBM7EEO8WOF/IMG_0310.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540952969-59QCV988XWKUM974WM64/IMG_0312.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540968777-5FSW5L3FDOT0AI7IKDC9/IMG_0337.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540970149-4A0DZLZNOP8P7TJO2OBD/IMG_0341.jpg Lab Blog - Christmas! https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1516540976464-YCTFKGU2SWDEFD7KHIUX/IMG_0343.jpg Lab Blog - Christmas! https://bose-lab.org/blog/2017/12/19/a-trip-back-to-philly monthly 0.5 2018-03-24 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569791264-9E0I5OF26X6VR9LHDHA9/IMG_0166.jpg Lab Blog - A trip back to Philly The Kaushal award prize-giving with the donor Dev Kaushal. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569622179-DLN75KIDYKFVMAQPN9OK/IMG_0165.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569622803-J9O340HVR65WOAX3CMQ1/IMG_0166.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569642806-W9WFRNECGR69X1MCOBHK/IMG_0169.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569666080-X2N7T00SXIG2K57TZWVZ/IMG_0175.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569666228-BV9DNYT4B4VQL7HJLVA0/IMG_0182.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569706349-EWLIAZUHC48SVGN1QS86/IMG_0186.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569708186-RM3NQC6S5B15W3G99CQE/IMG_0189.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569723649-ZZ3ZX8RLWOMQWH777CW4/IMG_0194.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569754559-46WV4LCMSM0VEYYFFDM4/IMG_0196.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569748862-TX9WHFBRAXQK4U2AHFHO/IMG_0204.jpg Lab Blog - A trip back to Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515569753998-YCZ88K3QWQYSJQR80C4Y/IMG_0205.jpg Lab Blog - A trip back to Philly https://bose-lab.org/blog/2017/12/19/some-new-faces-in-lab monthly 0.5 2018-01-09 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515484067350-JFHE4FXJLK9GH3DDFUSB/IMG_0273+%281%29.jpg Lab Blog - The best thing about starting a lab... https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515484063219-QVF3VQJUTBE373E230I6/IMG_0276+%281%29.jpg Lab Blog - The best thing about starting a lab... https://bose-lab.org/blog/2017/9/30/about-to-get-very-busy monthly 0.5 2018-01-09 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515483813282-F38YZ28XTYWNKSPPD547/IMG_0044.jpg Lab Blog - About to get very busy https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515483811816-L4XORD2KH955DYQZLTVO/IMG_0045.jpg Lab Blog - About to get very busy https://bose-lab.org/work-avenue daily 0.75 2023-04-11 https://bose-lab.org/new-gallery-1 daily 0.75 2023-04-11 https://bose-lab.org/schwane-avenue daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/56d4a125e707eb7a02e4055f/1456861631707-Q984I9AX2NYXST1ORUVB/3.jpg Schwäne https://images.squarespace-cdn.com/content/v1/56d4a125e707eb7a02e4055f/1456778213220-KRMLQULZ9K5DO2D19SFS/image-asset.jpeg Schwäne https://images.squarespace-cdn.com/content/v1/56d4a125e707eb7a02e4055f/1456778148407-LW8XWNCIRVQXOKWDMUAY/image-asset.jpeg Schwäne https://images.squarespace-cdn.com/content/v1/56d4a125e707eb7a02e4055f/1456778191954-MYS1Y99SFIHN5VE81TX9/image-asset.jpeg Schwäne https://bose-lab.org/about-us daily 0.75 2026-02-17 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493031751485-D1TPFBKSHA5L11JWCGB0/Firth-Court.jpg About https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604162164385-AN4PJDS25PB1LN4RZ17O/IMG_8697.jpeg About Firth court in the sunshine https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1544093376907-UT5UQYW3H2XUTWURB516/WT_logo-07.png About https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604169747359-HE1XS1LAUS0K8S2PF3JS/royal+society+logo+2.png About https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604169539572-PKC3BZ0BRSSEOMM15JPD/White+Rose+DTP.png About https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1604169561083-7X5ZR9KBZQ0LCF4K5DED/MRC+logo.png About https://bose-lab.org/research-details daily 0.75 2017-04-24 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493038023538-8G3QWXVTXO5X3U1RTSW9/Research-01.png Research https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493037542293-I4VGR7CG37TMUFJC233R/image-asset.png Research Figure 1: Enhancers and chromatin modifications. A) Enhancers are fundamental regulators of gene expression that operate far away from gene promoters, looping to contact their target genes. Different patterns of enhancer usage allow fine control of tissue and stimulus-specific gene expression patterns. B) In higher eukaryotes, DNA is packaged into a structure called chromatin. Tightly packed chromatin presents a barrier to gene transcription. However, chromatin structure can be regulated by post-translational epigenetic modifications of the histones that form nucleosomes around which DNA is wrapped. These modifications are controlled by a plethora of epigenetic-enzyme complexes to regulate gene expression. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493037589412-HKEATFYXYQPHO93ZT6G7/MBB+Figure-2.png Research Figure 2: How eRNA stimulates CBP activity. Our work demonstrated that eRNAs bind to a regulatory region within the catalytic acetyltransferase domain of the key transcription co-activator CBP. By displacing this region from the active site of the enzyme, eRNAs could stimulate the acetyltransferase activity and promote histone acetylation and transcription. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493041641564-67RM5WRVAOL2RTIH6GBQ/image-asset.png Research Figure 3: How do eRNAs affect enhancer activity? As the sequence and structure of eRNAs differs between enhancers, they are prime candidates to drive alternative profiles of enhancer activity to control gene expression. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493041664987-1X40949WM4S00VP9B5VG/image-asset.png Research Figure 4: How do eRNAs contribute to enzyme organization at enhancers? The flexible structures of epigenetic enzymes such as CBP allows them to adopt different conformations. We are investigating whether eRNA binding contributes to these distinct arrangements to promote engagement of the enzymes with chromatin at different enhancer regions. https://bose-lab.org/links daily 0.75 2023-04-13 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1681293211137-1DWYUXXSF6OVYFAQBUCO/IMG_6329.png Find out more https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493125884651-Z2IMHUGVQAF21U9RXBKV/image-asset.jpeg Find out more https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493040310985-F9WN3ZKVU5P2HYJAGWJU/image-asset.jpeg Find out more https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493040610869-5GP9NAJPZ8KZ2419OMCO/image-asset.jpeg Find out more https://bose-lab.org/people daily 0.75 2023-03-06 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1605535780646-ES5OKLXRDJFDO6HA6EKP/IMG_8504.jpeg People - Dan Bose Dan (and Ada) drinking coffee in Philly https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1572548968774-86GH15LLLNRU42TEZJU5/IMG_5666.jpeg People - Katie Gelder I started my PhD in the Bose Lab in October 2019. Previously, I completed an Integrated Master’s degree at the University of Sheffield studying Biochemistry and Genetics. As part of my Master’s year I completed a year in industry working at Eli Lilly, researching Alzheimer’s disease. I also previously undertook a summer research project at the University of Sheffield Medical School working with Professor Endre Kiss-Toth studying immunology. 2019 - present: PhD student, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK. 2018 - 2019: Master’s student in Industry, Eli Lilly, Erl Wood, Windelsham, Surrey. 2015 - 2019: Undergraduate student, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1573738719181-1YNDIZVYMIEUJ7SARQU1/IMG_5873.jpeg People - Nicola Carruthers I joined the Bose lab in October 2019 as a PhD student. Before this I completed my undergraduate degree in Biochemistry and Genetics, here in Sheffield. For my third year project I worked in Dr. Ewald Hettema’s lab where I studied protein orthologs in yeast. 2019 - present: PhD student, Department of Molecular Biology and Biotechnology, University of Sheffield 2016 - 2019: Undergraduate student, Department of Molecular Biology and Biotechnology, University of Sheffield https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1605280746187-GB4V8MTBQ2G6VZLO46NZ/IMG_3309.jpeg People - Laura Harrison I joined the Bose lab in October 2020 as a PhD student. Previously, I completed a BSc in Biochemistry and Genetics and an MSc in Human and Molecular Genetics at the University of Sheffield. During my MSc I studied the role of RNA-binding proteins in motor neurone disease and undertook a work placement in the oncology department at the Sheffield Children’s Hospital. Before starting my PhD, I worked as a laboratory technician at the University of Surrey. 2020 – Present: PhD student, Department of Molecular Biology and Biotechnology, University of Sheffield 2020: Laboratory Technician, Department of Nutritional Sciences, University of Surrey, Guildford, UK 2018- 2019: Postgraduate Taught student, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK 2015 – 2018: Undergraduate student, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1605118118694-X1ALANWPXK4QQGXUQ341/Lab+Website+Photo.jpg People - Molly John I joined the Bose Lab in October 2020 for my Master’s year, studying Biochemistry and Genetics. In my project I’m working on developing Cas13 systems for targeting eRNAs, hopefully giving a reliable method to study them and their role at enhancers. Previously I’ve worked on tagging proteins in a summer project in the Department of Chemistry, looked at mutations in malaria in Dr. Odom’s lab at WUSTL, and studied ribosomal proteins just next door with Dr. Emma Thomson in my 3rd year project. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1573741727990-TWHUHLUP2M7ZOFU019M1/IMG_5916.jpeg People - Luc Costello-Heaven I am a 4th year MBiolSci Molecular Biology student at Sheffield, working with the Bose Lab on developing nascent RNA sequencing with Oxford Nanopore Technology. I completed my 3rd year lab project in NMR with Prof. Mike Williamson and prior to this undertook a summer placement in the Cell Fate & Gene Regulation lab at the Francis Crick Institute. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1605088503482-ZQOI7300X9FF6FHT94LQ/IMG_9619.jpeg People - Monika Olejnik I joined the Bose lab in October 2020. For my MBiolSci degree, I am working on visualising transcription factor binding at enhancers using reconstituted nucleosomes and the magic of Electron Microscopy. I’ve been learning to tame proteins (with varying success) since my summer placement in Dr Bergeron’s lab back in 2018, and throughout my 3rd year research project in the Casson lab here in MBB. I enjoy afternoon walks in Bole Hills, preferably with a hot beverage in hand. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1573738842809-WN96RILA5Q397C3M0VYY/IMG_5665.jpeg People - Peyton Goddard, Georgie Hulme, Harry Heath Peyton, Harry and Georgie joined us in October 2019 to work on their 3rd year lab placement. For their project they are purifying histones and reconstituting chromatin arrays for functional assays and negative-stain electron microscopy. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543603129482-1DM69JYHWBKBZUM8UIDO/IMG_1880.jpg People - Christopher Callaghan Chris joined us for his 4th year MBiolSci project. He developed methods for probing RNA structure with single molecule FRET as part of our collaboration with Tim Craggs. Chris graduated in 2019 with a 1st class degree, and was awarded the Mike Fowler Medal for creative science! Since graduation, Chris has been working as a science teaching assistant; he plans to go on a journey of self-discovery in South East Asia in the new year. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1573739168697-0YONCP25OXVNF9WSEU4X/74634668_2595665360492259_1901784378770456576_n.jpg People - Naimah Hameed That post viva feeling - Naimah and bubbles https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1541596680893-JXQ8CIDFJGAVLCTCUMZ6/IMG_1237.jpg People - Sabina Musial Sabina joined us as on a highly competitive Genetics Society UK summer studentship at the end of her 1st year of undergraduate studies. The studentship also included a two day symposium in Edinburgh where Sabina presented her data. Since finishing her project, Sabina has been working on her 2nd year undergraduate course in MBB and has since completed a summer placement at the Karolinska Institute in Sweden. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1543603095065-KAPAB69QPP5OYYNY197N/IMG_1882.jpg People - Morgan Rustidge, Josh Zilate, James Taylor Morgan, James and Josh joined us for their 3rd year lab placement projects at the end of 2018. Morgan graduated in 2019 with a 2:1 honours degree and is now completing an MSc; James and Josh are completing 4th Year MBiolSci degrees. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1541596661172-0Z07NWRBUHUHE6QLYGQ0/IMG_1238.jpg People - Imogen Gatehouse Imo joined us as a SURE undergraduate rotation student in June 2018 at the end of her second year as an undergraduate in MBB. Since leaving the group, she has graduated with a 2:1 degree and is completing an MSc degree in Medical Genetics. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1515481546849-DB5WW289GDAWY5LPHAKM/IMG_0268+%281%29.jpg People - Christian Todaro Christian in lab. https://bose-lab.org/enhancers daily 0.75 2023-04-12 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493129127224-20LBEFN7GU7XP5KIWSK4/Enhancers-chromatin-05.png Enhancers and Chromatin Enhancer dependent gene regulation Enhancers are fundamental regulators of gene expression that operate far away from gene promoters, looping to contact their target genes. Different patterns of enhancer usage allow fine control of tissue and stimulus-specific gene expression patterns. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493129112645-V9XRGGMKDS2TVXPEDIVI/Enhancers-chromatin-06.png Enhancers and Chromatin Chromatin In higher eukaryotes, DNA is packaged into a structure called chromatin. Tightly packed chromatin presents a barrier to gene transcription. However, chromatin structure can be regulated by post-translational epigenetic modifications of the histones that form nucleosomes around which DNA is wrapped. These modifications are controlled by a plethora of epigenetic-enzyme complexes to regulate gene expression. https://bose-lab.org/epigenetic-enzymes daily 0.75 2021-09-03 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493129875331-7RHB1SDD4ZRJ97CD1U46/MBB+Figure-2.png Epigenetic Enzymes eRNA stimulates CBP activity. Our work demonstrated that eRNAs bind to a regulatory region within the catalytic acetyltransferase domain of the key transcription co-activator CBP. By displacing this region from the active site of the enzyme, eRNAs could stimulate the acetyltransferase activity and promote histone acetylation and transcription. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1497608008611-GLMADAYA7S4PO9871V0I/HAT-activity-03.png Epigenetic Enzymes Different eRNA sequences produce different patterns of CBP-dependent histone acetylation. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493130073656-D8WB2WEHG9VF459PGA6W/MBB+Figure-4.png Epigenetic Enzymes How do eRNAs contribute to enzyme organization at enhancers? The flexible structures of epigenetic enzymes such as CBP allows them to adopt different conformations. We are investigating whether eRNA binding contributes to these distinct arrangements to promote engagement of the enzymes with chromatin at different enhancer regions https://bose-lab.org/enhancer-rna daily 0.75 2023-04-12 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1493129969578-EYC64KKTYZP7HXMFT4KQ/MBB+Figure-3.png Enhancer RNA How do eRNAs affect enhancer activity? As the sequence and structure of eRNAs differs between enhancers, they are prime candidates to drive alternative profiles of enhancer activity to control gene expression. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1497607486205-VQU6RMW8Q8SX28TXWI5I/eRNAs-02.png Enhancer RNA CBP binds to eRNAs at enhancers. Left) UCSC genome browser view of CBP-bound eRNAs. Top; GROseq signal showing bi-directional eRNA transcription; Middle; CBP-bound RNAs from PAR-CLIP data; Bottom; Chromatin-bound CBP from ChIP-seq data. Right) CBP bound RNAs are enriched at transcribed enhancers: Top; Enrichment for CBP-bound RNAs (PAR-CLIP signal) at sites of CBP chromatin-binding (10th decile = most enriched for CBP); Bottom; Enrichment for CBP-bound RNAs (PAR-CLIP signal) at sites of nascent RNA transcription (GROseq signal, 10th decile = most enriched for nascent RNA). https://bose-lab.org/without-the-jargon daily 0.75 2024-01-31 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1497540603341-5LKRYD6GIP0ELJJM3U43/Join-us.jpg Without the jargon, please? Unravelling life's mysteries. https://bose-lab.org/roles-in-cancer-and-disease daily 0.75 2021-09-02 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1497626565489-QRNHGXJBHIBPTGWTG4GO/image-asset.png Roles in Cancer and Disease CBP domain model showing structures and disordered regions (A) and domain organization (B). C-D) Distribution of CBP mutations in cancer (cBioPortal, C) compared to mutiatons with no disease phenotype (ExAC, D); cancer mutations cluster within TF binding and catalytic domains. https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/1498059024235-0A9MLL02QBPI9PECVLKK/image-asset.png Roles in Cancer and Disease How do disease-related mutations at enhancers cause disease? One possibility is that they de-regulate the activity of epigenetic enzymes. https://bose-lab.org/new-page-1 daily 0.75 2023-04-11 https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/2aa95fad-9afc-43a7-8512-6573247a226a/CBP-blobs.png New Page https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/f0339162-137e-45ae-9428-6b63cb1ab7f3/eRNA_TAL1_AS_green_transb.png New Page https://images.squarespace-cdn.com/content/v1/58fdb54b579fb35db9fafd46/14c610f0-0936-4f0a-bd20-cf8111fe8280/screen-png.png New Page